There were dramatic scenes in Carndonagh this afternoon after a fire broke out in a barn.Large plumes of smoke were seen from across the town as the fire tore through the shed, which is located behind buildings near at Pound Street. Local reports say that the fire broke out at approximately 1pm.Fire services from Carndonagh attended the scene and quickly brought the blaze under control. No injuries were reported from the fire. However, the barn was extensively damaged.See footage from the incident by VIDEOTEK MEDIA PRODUCTIONS here: Fire crews attend Carndonagh barn blaze was last modified: October 2nd, 2019 by Rachel McLaughlinShare this:Click to share on Facebook (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Click to share on Reddit (Opens in new window)Click to share on Pocket (Opens in new window)Click to share on Telegram (Opens in new window)Click to share on WhatsApp (Opens in new window)Click to share on Skype (Opens in new window)Click to print (Opens in new window)
Nuno disappointed with Wolves wastefulness for Braga defeatby Paul Vegasa month agoSend to a friendShare the loveNuno was disappointed in Wolves’ wastefulness in the 1-0 loss to Braga on Thursday night.Patrick Cutrone, Ruben Neves and Willy Boly all had chances to opening the scoring for the hosts, but it was ultimately Ricardo Horta’s 72nd-minute strike that sealed a win for the Portuguese club.”We are all disappointed. It was a tough game, but I think we did enough,” Nuno said.”We did not perform perfectly, but we created many chances.”Braga had one chance, were very organised, and they scored.”That is the story of the game.”The boys worked hard, but that is football.” About the authorPaul VegasShare the loveHave your say
Story Highlights The Ministry of Health is in the process of finalising a report from a review of the four regional health authorities, which will be the subject of stakeholder consultations during the 2018/19 fiscal year. The review, which was led by a team from the Pan American Health Organization (PAHO), looked at the authorities’ structure and performance, and proposes specific reforms which will be pivotal to the Government’s strategic plan for the public health sector. Dr. Tufton said the strategic plan is aimed at advancing the sector over the next 10 to 30 years through the completion of a number of critical supporting studies and reports that address several key areas. The Ministry of Health is in the process of finalising a report from a review of the four regional health authorities, which will be the subject of stakeholder consultations during the 2018/19 fiscal year.The review, which was led by a team from the Pan American Health Organization (PAHO), looked at the authorities’ structure and performance, and proposes specific reforms which will be pivotal to the Government’s strategic plan for the public health sector.This was announced by Portfolio Minister, Dr. the Hon. Christopher Tufton, during his 2018/19 Sectoral Debate presentation in the House of Representatives on Tuesday (June 5).Dr. Tufton said the strategic plan is aimed at advancing the sector over the next 10 to 30 years through the completion of a number of critical supporting studies and reports that address several key areas.Another component of the plan, the Minister informed, is the commissioning of a needs assessment study for five hospitals, which is being conducted by the United Nations Office for Project Services (UNOPS).The beneficiary institutions are the Spanish Town, May Pen, Mandeville, St. Ann’s Bay and Kingston Public hospitals.The study will provide recommendations for the facilities’ upgrading and/or expansion to determine how best the healthcare infrastructure can be networked and overhauled to address future challenges.“These studies will… make a determination as to how we operate (in terms of, for example) where we add beds and operating theatres in order to optimise the infrastructure that is available. We are deficient now, and this will add some value to that process. These (studies) are going to be critical to the (strategic) plan, and we are hoping that by mid-year, we will have the framework of that plan,” he stated.Dr. Tufton advised further that a report on the status of the sector’s information systems has also been completed. The document, which was facilitated under a technical cooperation agreement with the PAHO/World Health Organization (WHO), will form part of the strategic plan.Meanwhile, a road map for healthcare financing has been established and a report on the status of the national health insurance scheme, in this regard, is being completed. The report is expected to be ready within two months.
Senior running back Carlos Hyde (34) walks of the field during a game against Illinois Nov. 16 at Memorial Stadium. OSU won, 60-35.Credit: Ritika Shah / Asst. photo editorLives have changed forever for 256 players as they were selected in the 2014 NFL Draft.Six of those players are former Ohio State Buckeyes.The first former Buckeye taken off the board was linebacker Ryan Shazier, who was selected 15th overall by the Pittsburgh Steelers in the first round Thursday night. He became the ninth former OSU linebacker and 68th Buckeye ever taken in the first round.Shazier, who was a first-team Associated Press All-American in his final season at OSU, totaled a Big Ten conference-high with 143 tackles to go along with six sacks.Cornerback Bradley Roby, who joined Shazier as one of 30 prospects invited to New York for the draft, was selected by the Denver Broncos with the 31st overall pick in the first round.Roby became the first OSU defensive back taken in the first round of the draft since Malcolm Jenkins was selected by the New Orleans Saints with the 14th overall pick in 2009.The Broncos, who dropped Super Bowl XLVIII 43-8 to the Seattle Seahawks on Feb. 2, have since bolstered their secondary by not only drafting Roby, but also signing former New England Patriots cornerback Aqib Talib to a six-year, $57 million contract in March.Just as they did in the first round, the Buckeyes produced two picks in the second round, as running back Carlos Hyde and offensive lineman Jack Mewhort came off the board.Hyde was selected 57th overall by the San Francisco 49ers, who are coached by former University of Michigan quarterback Jim Harbaugh.The former Buckeye running back became the first 1,000-yard rusher under OSU coach Urban Meyer and was named the Big Ten Ameche-Dayne Running Back of the Year in 2013. Hyde finished his senior campaign with 1,521 yards and 15 touchdowns on the ground.Mewhort was selected just two picks later with the 59th overall pick by the Indianapolis Colts.The selection of Mewhort marked the third straight year that an OSU tackle has been selected in the draft, following in the footsteps of Reid Fragel – drafted by the Cincinnati Bengals in 2013 – and Mike Adams, who the Pittsburgh Steelers selected in 2012.Mewhort was a three-year starter in his time as a Buckeye and was named a first-team All-American by ESPN.com during his senior season in 2013.Another Buckeye was not taken until the fifth round when center Corey Linsley was selected 161st overall by the Green Bay Packers.Linsley anchored an offensive line that ranked fifth nationally in 2013 in rushing, averaging 308.6 yards per game and breaking school records for points in a single season with 637, as well as touchdowns scored with 87.Linsley graduated from OSU with a degree in economics in December.Rounding out the former Buckeyes selected in the 2014 draft was safety Christian Bryant, who was selected 241st overall in the seventh round by the St. Louis Rams.Bryant, who was named a team captain prior to the 2013 season, suffered a season-ending ankle injury during the fifth game of the year against Wisconsin Sept. 28., a game won by OSU 31-24. At the time of the injury, Bryant ranked third on the team in tackles with 22.Bryant, who was a three-year starter for the Buckeyes, finished his OSU career with 171 total tackles to go along with two interceptions, 23 pass breakups and three forced fumbles.The Buckeye safety petitioned the NCAA for a medical redshirt in January, but was turned down and entered the draft.Although six Buckeyes were drafted to NFL rosters, several draft-eligible players were not selected during the draft.According to multiple reports, many of the former Buckeyes not drafted have found homes in free agency.Some of the players signing in free agency took to Twitter to announce their new teams, one of them being wide receiver Corey “Philly” Brown who announced his signing with the Carolina Panthers saying, “Here I come Carolina,” via his personal account @phillybrown10. Reportedly joining him in Carolina will be offensive lineman Andrew Norwell.Offensive lineman Marcus Hall confirmed with The Lantern Sunday in an email that he has signed with the Indianapolis Colts, which reunites him with Mewhort. Safety C.J. Barnett’s signing with the New York Giants was confirmed via Twitter by his signing agency IFA.Kicker Drew Basil also took to Twitter late Saturday night announcing he has signed with the Atlanta Falcons. Wide receiver Chris Fields also used social media to announce an agreement with the Washington Redskins.Running back Jordan Hall has earned a tryout with the Steelers according to multiple reports and quarterback Kenny Guiton remains unsigned as of Sunday morning.
Unlike Cristiano Ronaldo and Lionel Messi, the young rising star in Kylian Mbappe “couldn’t care less” about the Ballon d’Or award following a brilliant World Cup campaignThe 19-year-old is being tipped as a dark horse to put an end to Ronaldo and Messi’s dominance of the highly-coveted award this year and may well in with a shot, depending on the final outcome of France’s World Cup campaign.Mbappe’s meteoric rise in the football world has been undeniable following his standout performances at Russia, which even left a five-time Ballon d’Or winner in Messi in the shadows after the Paris Saint-Germain inspired France to a 4-3 over Argentina in the last-16.While many had expected his illustrious PSG teammate Neymar to be the one to end Ronaldo and Messi’s decade-long dominance of the Ballon d’Or, it appears that it could be Mbappe instead.But it winning the historic award, that has been awarded to greats like Michel Platini and Marco van Basten since it was introduced in 1958, is something that doesn’t seem so appealing to the young man.Fiorentina owner: “Ribery played better than Ronaldo!” Andrew Smyth – September 14, 2019 Fiorentina owner Rocco Commisso was left gushing over Franck Ribery’s performance against Juventus, which he rates above that of even Cristiano Ronaldo’s.“I couldn’t care less about that,” he told TF1, via Express.“I want the World Cup. I want to sleep with it.“I wasn’t born in 1998, so I have never seen people on the Champs-Elysees celebrating.Mbappe has been linked with a move to Real Madrid to replace Ronaldo after his exit to Juventus on Tuesday.
The Argentine Football Association (AFA) have confirmed that head coach Jorge Sampaoli has vacated his post following Argentina’s disappointing World Cup campaignAfter two shock results against Iceland and Croatia, the South American side barely managed to scrape through the group stages of the competition before falling to eventual champions France in the Round of 16 with a 4-3 defeat.While Sampaoli had only been appointed Argentina boss in May 2017, there were reports that had emerged during the World Cup that had suggested that he was following Lionel Messi’s orders.Top 5 best players from the international break weekend Tomás Pavel Ibarra Meda – September 11, 2019 After a fresh international break just came to an end, we need to talk about the Top 5 best players during this whole weekend.We…Now the 58-year-old has had his contract at Argentina mutually terminated with the AFA.“The Argentine Football Association [AFA] and the ex-coach of the national team Jorge Sampaoli today reached a mutual agreement to rescind his contract,” read the statement on Sky Sports.Argentina had their worst World Cup campaign this summer since 2002, which saw them being eliminated from the group stages at Japan and South Korea.
AC Milan coach Gennaro Gattuso hopes Gonzalo Higuain will remain calm following his sending off against Juventus on SundayThe 30-year-old striker has enjoyed a good start to life on loan at Milan from Juventus with seven goals in 13 games.However, Higuain was unable to prevent Milan from losing to his parent club at the San Siro and was dismissed after having a penalty saved.The Argentine was handed a second yellow card after shouting in the match official’s face.But Gattuso believes that Higuain’s angry outburst came about due to putting too much pressure on himself.Corini tells Balotelli to “raise his game” Manuel R. Medina – September 14, 2019 According to the Brescia coach, Mario Balotelli “needs to raise his game if he wants to face Juventus” as his team is set to host Bologna.“At this moment he feels a lot of pressure, my words from yesterday’s press conference were not by chance,” Gattuso told Milan TV on the club website.“Right now, he feels a huge weight on him and it shows. He must remain calm, he should apologise and assume his responsibilities.“He has to put aside his nervousness, he is the most important player we have and he has to make the difference. He has to be clear-headed.”Mario Mandzukic and Cristiano Ronaldo were the goalscorers for Juventus, who remain unbeaten after 12 Serie A games.
(Representational Image)Creative CommonsA Trichy auto driver was beaten to death by a group of intoxicated men after he tried stopping them from harassing his female passenger. The incident happened on May 19, Sunday, outside the Trichy Famous Biryani restaurant.The victim, identified as Abdullah, had taken his passenger to Trichy Famous Briyani restaurant. He waited for her outside the restaurant while she went inside to pick her parcel. During that time, an inebriated man bumped into the woman on purpose, revealed Tashmin Banu, the victim’s wife.”My husband immediately questioned the man and the people with him, and then, a tussle ensued. They hit each other and then the drunk man left, threatening to bring more people. But my husband didn’t take it seriously. However, he did come back with three men and they all hit him till he died. They used a wooden plank to beat his head,” Tashmin told The News Minute.However, when the Srirangam Assistant Commissioner of Police, K Ramachandran, was contacted, he brushed off the incident, saying that Abdullah was also inebriated and got into a fight with the other men who were also drunk.”Abdullah was also inebriated and he died because he was pushed to the ground and sustained head injuries. It was not a big fight…The man who allegedly harassed this woman is Nagarajan, who was also drunk,” Ramachandran said.Tashmin, on the other hand, is very confident that her husband would never drive when he is intoxicated. She questioned why the police were saying that he too was drunk. She said, “All he did was fight against people harassing a woman and there is nothing wrong in that. Not even one person in that restaurant came to help him and now the police are saying this.”
Complete destruction of Rohingya villages in close proximity to intact Rakhine village, Maungdaw township, recorded on 21 September 2017. Photo: Human Rights WatchHuman Rights Watch (HRW) on Tuesday reported destruction of tens of thousands of structures in Myanmar, which are primarily homes inhabited by ethnic Rohingya Muslims.Referring to some newly released satellite images, the rights group said at least 288 villages were partially or totally destroyed by fire in northern Rakhine state since 25 August 2017.HRW analysis says the burnings took place after the Myanmar officials claimed security force “clearance operations” had ceased.The images show at least 66 villages were burned after 5 September, when security force operations supposedly ended, according to a 18 September speech by state counselor Aung San Suu Kyi.The Myanmar military responded to reported attacks on 25 August by the Arakan Rohingya Salvation Army (ARSA) with a campaign of ethnic cleansing, prompting more than 530,000 Rohingya to flee across the border to Bangladesh, according to the United Nations refugee agency.“These latest satellite images show why over half a million Rohingya fled to Bangladesh in just four weeks,” said Phil Robertson, deputy Asia director. “The Burmese military destroyed hundreds of Rohingya villages while committing killings, rapes, and other crimes against humanity that forced Rohingya to flee for their lives.”A total of 866 villages in Maungdaw, Rathedaung, and Buthidaung townships in Rakhine state were monitored and analysed by Human Rights Watch.The most damage occurred in Maungdaw township, accounting for approximately 90 per cent of the areas where destruction happened between 25 August and 25 September.Approximately 62 per cent of all villages in the township were either partially or completely destroyed, and southern areas of the township were particularly hard hit, with approximately 90 percent of the villages devastated.In many places, satellite imagery showed multiple areas on fire, burning simultaneously over wide areas for extended periods.Images showed that most of the damaged villages were 90 to 100 per cent destroyed.Many villages which had both Rohingya and Rakhine residing in segregated communities, such as Inn Din and Ywet Hnyo Taung, suffered heavy arson damage from arson attacks, with known Rohingya areas burned to the ground while known Rakhine areas were left intact.The Myanmar government has repeatedly said that ARSA insurgents and local Rohingya communities were responsible for setting the fires that wiped out their villages, but has offered no evidence to support such claims.Human Rights Watch interviews in Bangladesh with more than 100 refugees who had fled the three townships gave no indication that any Rohingya villagers or militants were responsible for burning down their own villages.HRW insisted that UN member countries and international bodies should press the Myanmar government to grant access to the UN-mandated fact-finding mission to investigate these abuses.The UN Security Council should also urgently impose a global arms embargo on Myanmar , and place travel bans and asset freezes on those Myanmar commanders responsible for grave abuses, the rights group said.”Governments should impose a comprehensive arms embargo against Burma (Myanmar), including prohibiting military cooperation and financial transactions with military-owned enterprises.”“The shocking images of destruction in Burma (Myanmar) and burgeoning refugee camps in Bangladesh are two sides of the same coin of human misery being inflicted on the Rohingya,” Robertson said. “Concerned governments need to urgently press for an end to abuses against the Rohingya and ensure that humanitarian aid reaches everyone in need.”
Dr. Wadie F. Bahou, MD, in Stony Brook’s Department of Medicine, works with a research group consisting of Varsha Sitaraman and Dmitri V. Gnatenko in the Department of Medicine; Patrick Hearing, Eckard Wimmer and Steffen Mueller in the Molecular Genetics and Microbiology Department; and Steve Skiena and Charles B. Ward in the Computer Sciences Department.“Our group is composed of individuals with long-standing interests in computational biology, virology, and gene therapy using viruses,” says Bahou. Thus, my group has been most interested in modifying genomes as strategies for gene therapy, Eckard and Steffen are pioneers in recoding viruses for vaccine development, while Steve and Charles provide the critical common thread for all our computational studies.”The key step in their achievement was to computationally redesign Rep 78/68 so that it was better tolerated by adenoviral vectors. “Prior research by our team had previously developed a computational algorithm for redesigning genes based on synonymous codon pair recoding,” Bahou notes. “The real leap of faith was to ask whether the algorithm could be applied to the case of viral/viral interactions. The redesign was relatively straightforward using the previously developed computational algorithm.”The methods that the team used to demonstrate that their leap of faith was justified were equally innovative. As Bahou explains, “The real innovation here is progressive evidence that synonymous pair bias actually can be manipulated to change fundamental characteristics of biological functions. Members of our team have previously demonstrated this for poliovirus and influenza virus, and in this case, we have shown its relevance for generating a novel gene therapy adenovirus.”Bahou also sees a number of potential avenues for further developing the group’s line of research. “Certainly the possibility of using these reengineered viral systems for vaccine development is an obvious application. For applications directly related to this most recent manuscript,” he continues, “it provides a unique opportunity for safe methods for long-term gene delivery. On a broader application, one could envision entire cell systems with recoded genes as a means of altering cellular function in general. This is a forward-thinking statement, but certainly predicated on the status of genome synthesis coupled with progressive information on genetic pathways that regulate cell functions.”Those applications, he adds, will most likely feature the ability to rapidly characterize and synthesize attenuated genomes as new vaccines. In addition, “we are currently testing our ability to safely use this technology for gene replacement strategies which have been limited by concerns for insertional mutagenesis” – mutations caused by inserting genes into a genome. “Such an issue is especially relevant for benign diseases such as hemophilia where cellular transformation using integrating lentiviruses should be considered an unacceptable risk for long-term gene replacement strategies. Therefore,” Bahou points out, “our collective group is currently trying to apply this research as a safe means of long-term gene replacement. At the same time, Bahou stresses that “It’s important to note that genome reengineering using synonymous codon pair restructuring is a relatively new concept, and we fully anticipate that the applications will broaden over time. In theory,” he concludes, “we should be able to modify genomes for both attenuated expression and enhanced expression, depending on the situational requirements. In that respect, the technology is relevant for many other biological systems, especially when developing computational approaches to modifying cellular systems at many levels.” Viruses evolve to play by host rules Citation: Genes ex silico: Computer-designed virus yields phenotype expression benefits (2011, August 29) retrieved 18 August 2019 from https://phys.org/news/2011-08-genes-silico-computer-designed-virus-yields.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. More information: Computationally designed adeno-associated virus (AAV) Rep 78 is efficiently maintained within an adenovirus vector, Published online before print August 15, 2011, doi:10.1073/pnas.1102883108 , PNAS August 23, 2011 vol. 108 no. 34, 14294-14299 Functional characterization of single-backbone Ad/AAV/dRep hybrid virus. (A) Schema of Ad/AAV/dRep with predicted monomeric (M) and dimeric (D) forms resulting from productive Rep 78 cleavage of endogenous AAV integrating elements p5IEE and AAV TR (Fig. 2 shows excision model). Sizes are as follows: ψ, Ad packaging sequence (0.4 kb); pCMV/EGFP cassette (1.6 kb); pTK/NeoR, (1.1 kb); Ad, Ad base pairs 3,330 to 3,940 (0.6 kb); p5IEE, 135 bp; AAV TR (145 bp each plus G-C tail); and dRep, approximately 1.9 kb. (B) Ad/AAV/dRep was serially passaged in 293 cells, and at indicated passages (P), Hirt DNA was isolated for genomic analysis using the approximately 0.2-kb Ad left end inverted TR (ITR base pairs 1-194) as probe (horizontal bar). *Parent Ad/AAV/dRep virus. © PHAS, doi: 10.1073/pnas.1102883108 (PhysOrg.com) — Gene therapy is medicine’s rising star with adeno-associated virus (AAV) vectors – nonpathogenic parvoviruses – among the most promising supporting actors, due largely to their capability to integrate into transcriptionally silent genomic regions (areas that do not, via RNA polymerases, make a messenger RNA copy of DNA-stored genetic information). That being said, there’s been a downside in assembling AAV vectors into adenoviral (Ad) viral backbones, which are used extensively in genetic research and therapy: They rely on replication (Rep) proteins – in this study, the Rep 78/68 polypeptide – which limit viral amplification methodologies. Recently, however, researchers at Stony Brook University have used computational microbiology to reengineer the Rep gene through synonymous codon pair recoding, a technique in which the meaning of a codon (a set of three base nucleotides) is changed in a dynamic site- or mRNA-specific way – that is, it competes and shares expression with the standard. The experimental results demonstrate the value synonymous codon pair reengineering can have in modifying endpoint expression. Copyright 2011 PhysOrg.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed in whole or part without the express written permission of PhysOrg.com. Explore further