PSG chief Henrique casts suspicion on Barcelona over Rabiot behaviourby Carlos Volcano10 months agoSend to a friendShare the lovePSG chief Antero Henrique has hit out at Barcelona over their attempts to sign Adrien Rabiot.Rabiot has been banished to the stands for refusing to consider new contract talks at PSG, where his current deal expires in June.Henrique suspects the influence of Barca and snapped: “Mrs. Rabiot said that we had received an offer this summer. That’s right, a bid on August 29, twenty hours before the end of the transfer window. “It is then simple: Barcelona first had to reach an agreement with Rabiot before we were being talked to. And you also need to know that the Barcelona offer was ridiculous.”We, the club, were surprised by the low bid. It was disrespectful for someone with the status of Rabiot. I continue to emphasise: it is not possible to reach agreement within 24 hours on a player like Rabiot. It seems clear to me that Barcelona already had an agreement with Adrien. “I’m sure he has an agreement with another club anyway. Whether he can leave in the winter? He told me he does not want to stay.” TagsTransfersAbout the authorCarlos VolcanoShare the loveHave your say
Nuno disappointed with Wolves wastefulness for Braga defeatby Paul Vegasa month agoSend to a friendShare the loveNuno was disappointed in Wolves’ wastefulness in the 1-0 loss to Braga on Thursday night.Patrick Cutrone, Ruben Neves and Willy Boly all had chances to opening the scoring for the hosts, but it was ultimately Ricardo Horta’s 72nd-minute strike that sealed a win for the Portuguese club.”We are all disappointed. It was a tough game, but I think we did enough,” Nuno said.”We did not perform perfectly, but we created many chances.”Braga had one chance, were very organised, and they scored.”That is the story of the game.”The boys worked hard, but that is football.” About the authorPaul VegasShare the loveHave your say
LOS ANGELES, CA – DECEMBER 03: head coach John Calipari of the Kentucky Wildcats reacts to a foul against his team during the first half against the UCLA Bruins at Pauley Pavilion on December 3, 2015 in Los Angeles, California. (Photo by Harry How/Getty Images)Kentucky pulled out a big win at LSU tonight to push its record to 24-0, but if you believe John Calipari, there were times tonight when he would have welcomed 23-1. Karl-Anthony Towns’ was called for a technical for hanging on the rim midway through the second half, which led to this amazing post-game moment on ESPN. As much fun as that videobomb is, Calipari was livid about the foul, at that point in the game. In the interview he said that he told the team that he hoped they would lose after the foul, and reiterated that point to open his post-game press conference. Calipari goes on to praise his talented freshman as a player and a person, but clearly this mistake, which could have proven incredibly costly in what wound up being a two point game, really bothered him.Kentucky hosts South Carolina this Saturday at 2 p.m.
TORONTO – A lawsuit launched by a controversial Toronto mayoral candidate against Bell Media after the broadcaster refused to air her campaign advertisement on a local television station has been dismissed by an Ontario Superior Court justice.In his written decision, Justice Peter Cavanagh said Faith Goldy’s complaint should be addressed to the Canadian Radio-television and Telecommunications Commission and not the court.Goldy, a former journalist who is now a fringe candidate in Toronto’s municipal election, wanted the court to order the broadcaster to run the ad until the Oct. 22 vote.She alleged that Bell Media broke national broadcasting rules and breached her right to free expression in refusing to air her campaign advertisement.Cavanagh said that while the court has jurisdiction when it comes to charter rights issues, he decided not to deal separately with those matters in this case.“I decline to exercise my discretion to carve out the charter issues and have them heard separately by the Superior Court of Justice,” Cavanagh said in his decision. “The entire application should be heard by the same tribunal, the CRTC.”Goldy, whose campaign includes anti-immigrant policies, once penned an article suggesting there was “white genocide” in Canada. “Vote Faith Goldy for mayor and Toronto ceases to be a Sharia safe space,” she said in a recent tweet.Bell Media told the court that it had received over 80 written complaints and requests from a number of groups not to run Goldy’s ads. It called the move a business decision.The company said it would not be commenting further on the case.Cavanagh said the fact Goldy did not attempt to have the CRTC hear her complaint and her overall standing in the mayoral race were factors in his decision.“I also consider that Ms. Goldy is polling at approximately six per cent according to the submissions of her own counsel and that the outcome of this application will not have any realistic impact on the outcome of the race,” he said.In documents filed last week, Goldy alleged Bell Media initially agreed to air her campaign ad on its television station CP24 and accepted a deposit of more than $13,000.She alleged the broadcaster later refunded her money and refused to run the clip, without providing an explanation for its decision.Her lawyer, Clayton Ruby, said last week there is nothing in the ad itself that the broadcaster could object to, calling it “utterly innocuous.”Ruby did not immediately respond to request for comment on Tuesday.Goldy also stirred controversy last month after posing for a photograph with Premier Doug Ford, prompting him to distance himself from her several days later following questions from the opposition.
OSU junior linebacker Raekwon McMillan (5) dances towards the crowd after the Buckeye defense stops the Nebraska offense on their own two-yard line during the first half of the game on Nov. 5. The Buckeyes won 62-3. Credit: Alexa Mavrogianis | Photo EditorRaekwon McMillan came to Ohio State as one of the most highly rated recruits in the 2014 class. Like any other freshman, McMillan, a Georgia native, was getting acclimated with classes, new teammates and a new scenery. Two years later, McMillan is the face of the OSU defense, the Silver Bullets, and figures to be the next in the lineage of OSU linebackers in the NFL.The junior middle linebacker has been the soul of the defense for the Buckeyes, but also this fall, he began to exercise his political freedom. McMillan became involved in voter registration in central Ohio for the 2016 election season as a member of the Ohio State chapter of Omega Psi Phi fraternity.“We had a program of voter registration and really just spread the word, mostly to the African-American people throughout central Columbus,” McMillan said. “That’s what we try to focus on, but we just want to build awareness and teach what this election is about, what each candidate stands for.”Omega Psi Phi is a historically African-American Greek life organization that is governed by the National Pan-Hellenic Council. Redshirt sophomore safety Damon Webb also is a member.McMillan joined the fraternity after his sophomore year. At first, he said he never really saw himself as a fraternity member, however he began to look more into the extracurriculars, which drew him to Omega Psi Phi. McMillan said that, before the 2016 election cycle, he never really into politics, but began to pay more attention to it as the presidential campaign intensified. As an “Omega man,” McMillan felt like it was his duty to encourage others to take political action in an unprecedented election.OSU coach Urban Meyer hosted Patriot Week throughout the Memorial Day weekend this past spring, bringing in several guest lecturers to talk to the team about their responsibilities as Americans — voting being one of them. On Monday, Meyer expressed how important it is to him that his team understands the civic duties each individual has, and said he hopes each of his players votes on Tuesday. McMillan, along with redshirt junior quarterback J.T. Barrett, said they will vote on Tuesday.“It’s something that’s going to impact us not only here in Columbus but also around the country,” McMillan said. “So we want everybody to have awareness to what’s going on around us because you’re exercising your political rights.”Webb said McMillan’s activism in Omega Psi Phi has a direct correlation with the linebacker’s ability to persevere in intense situations and uplift his teammates. Webb added that the leadership traits McMillan are not confined to the gridiron.“I would definitely say he’s gotten better. He’s always been a leadership-type dude, so even outside of the football field — our fraternity brothers, he brought leadership to them,” Webb said. “It’s like a brotherhood outside of the locker room … we build a bond that is unbreakable.”
Barcelona’s Sergio Busquets has reached an agreement with the club over a new deal, according to Marca.The club’s vice-captain is closing in on signing a new contract that would see him increase his salary at the club. Busquets signed an extension recently in 2016 and didn’t demand for a new pay rise but the club hierarchy felt the midfielder has earned the right to earn more money at the Camp Nou.The new contract isn’t really an extension for the Spaniard but an improvement in terms of his wages with the length of the contract remaining the same.The 2019/2020 betting odds for La Liga Stuart Heath – August 12, 2019 Going into La Liga kick-off, we take a look at what the bookmakers think.La Liga season is less than a week away and we’re…It runs until June 2021, although Barcelona retain a club option to extend that to the summer of 2023. Busquets’ buy-out clause stands at €200million, a figure that is rather low when you consider the money spent by the Blaugrana on inferior midfielders as recently as the January transfer window.Sergi Roberto’s own contract renewal was finalised in January and this had an embedded buy-out clause of 500 million euros.Simply, the Catalan giants don’t believe Busquets will ever truly push to leave, and this aforementioned bump in pay puts the holding midfielder into the second tier of Barcelona’s squad just below Lionel Messi.
Ed Lenderman Categories: Good Morning San Diego, Local San Diego News July 16, 2018 00:00 00:00 spaceplay / pause qunload | stop ffullscreenshift + ←→slower / faster ↑↓volume mmute ←→seek . seek to previous 12… 6 seek to 10%, 20% … 60% XColor SettingsAaAaAaAaTextBackgroundOpacity SettingsTextOpaqueSemi-TransparentBackgroundSemi-TransparentOpaqueTransparentFont SettingsSize||TypeSerif MonospaceSerifSans Serif MonospaceSans SerifCasualCursiveSmallCapsResetSave SettingsCHULA VISTA (KUSI) – The long-awaited makeover of Chula Vista’s bayfront now includes some 25 double-trailer dump trucks going up and down the I-5 between Chula Vista and the UC-San Diego campus.It’s a welcome sight for Chula Vista and Port District Officials, as well as Chula Vista residents. The site, is where the center piece of the 500-acre bayfront makeover is being built: A 1,600 room resort hotel and adjoining convention center, 275,000 square feet.And starting early Monday morning and continuing all day long, through August 11th, this will be the scene here– truckload after truckload of dirt being hauled in from an excavation project at the UC San Diego campus.The goal is to build up the site some 6 to 8 feet– that takes some 210,000 cubic yards of earth. Hence, the convoy of big rigs up and down the five through the middle of August.Engineers are increasing the site’s elevation for two reasons– drainage would be an obvious one, but with an eye on the future– the second reason is the continued warnings about future sea-level rise.The port’s project manager, Mark McIntire, said the Port has been studying this issue for a long time and through science and theoretical projections, determined there is a lot of need to raise sites near the coastline various elevations. They’re also raising another nearby area, using 60,000 cubic yards of earth. Posted: July 16, 2018 Chula Vista Bayfront makeover construction continues Ed Lenderman, FacebookTwitter
For additional information call 907-283-8262 or 907-283-8235. All volunteers will meet at the Kenai Public Library, and maps will be provided upon arrival. The next event is scheduled for Thursday, October 4, beginning at the corner of Fourth Avenue and Spruce Street. The first event took place on August 30, and volunteers collected four trash bags with garbage along Ryan’s Creek Trail greenbelt. Each hike will be from noon to 1pm, at Ryan’s Creek Trail, and is family friendly. The city will provide bags, gloves, and water. This event is hosted by the City of Kenai Parks & Recreation: “Use your lunch break to experience Kenai’s parks, exercise, and extinguish trash” Facebook0TwitterEmailPrintFriendly分享The City of Kenai will be hosting the second in a series of ‘TRASHercize Hike’ events on Thursday, September 20.
Annette March-Grier, executive director of Roberta’s House in Baltimore.The executive director of a Baltimore-based grief and loss counseling center was selected as one of 10 finalists for CNN’s Hero of the Year, a worldwide competition honoring those who give back to their community.Annette March-Grier, executive director of Roberta’s House in Baltimore, was announced as one of CNN’s Top Ten Heroes on Oct. 2“I was really awestruck and surprised; I did not imagine that Roberta’s House would make it to the top ten,” March-Grier told the AFRO. “I was humbled in the fact that CNN actually felt as though the work that we do was significant enough that it should be recognized for such a status.”The yearly broadcast of “CNN Heroes: An All-Star Tribute,” highlights everyday people who are changing their communities and the world. In Baltimore, March-Grier has been an advocate for addressing the damaging effects that losing a loved one can have on the lives of individuals.Roberta’s House hosts seven programs for adolescents and adults suffering with grief. The center is a safe space serving more than 500individuals each year who learn how to express themselves in healthy ways, develop positive coping skills, learn lessons behind loss and create memories of their loved ones to empower their futures.Outside of the community, the organization touches at least 1,000 more people each year through its summer camp and support training programs for churches and professionals. Launched this year, the Survivor Advocacy Program trains individuals who make visits within 24 to 48 hours after a homicide to provide grief counseling and other resources to related victims.“Being able to see people transform in just a short period of time from being depressed, hurt and angry to finding a desire to live and grow with positive memories is the greatest reward,” said March-Grier.As a finalist for the Hero of the Year honor, March-Grier will receive a $25,000 grant and free organizational training from the Annenberg Foundation, including practical guidance in fundraising, communications and management of volunteers.If she wins the CNN Hero of the Year, Roberta’s House will receive a $100,000 award—money she said will be used as seed funding for a brand new center that will be developed over the next three years. The winner is selected by an online vote.“By voting, we all can take action in making a difference,” said March-Grier. “This is an opportunity where everyone in Baltimore can play a role in supporting the work that we do—we all, as a community, have to take charge in healing our community.”Voting for CNN Hero of the Year can be accomplished at www.CNNHeroes.com and via the CNN app through Sunday, Nov. 16 at midnight Pacific. “CNN Heroes: An All-Star Tribute” will air on Sunday, Dec. 7 at 8:00 p.m. Eastern on CNN, and will be simulcast on CNN International and CNN en Español.
Dr. Wadie F. Bahou, MD, in Stony Brook’s Department of Medicine, works with a research group consisting of Varsha Sitaraman and Dmitri V. Gnatenko in the Department of Medicine; Patrick Hearing, Eckard Wimmer and Steffen Mueller in the Molecular Genetics and Microbiology Department; and Steve Skiena and Charles B. Ward in the Computer Sciences Department.“Our group is composed of individuals with long-standing interests in computational biology, virology, and gene therapy using viruses,” says Bahou. Thus, my group has been most interested in modifying genomes as strategies for gene therapy, Eckard and Steffen are pioneers in recoding viruses for vaccine development, while Steve and Charles provide the critical common thread for all our computational studies.”The key step in their achievement was to computationally redesign Rep 78/68 so that it was better tolerated by adenoviral vectors. “Prior research by our team had previously developed a computational algorithm for redesigning genes based on synonymous codon pair recoding,” Bahou notes. “The real leap of faith was to ask whether the algorithm could be applied to the case of viral/viral interactions. The redesign was relatively straightforward using the previously developed computational algorithm.”The methods that the team used to demonstrate that their leap of faith was justified were equally innovative. As Bahou explains, “The real innovation here is progressive evidence that synonymous pair bias actually can be manipulated to change fundamental characteristics of biological functions. Members of our team have previously demonstrated this for poliovirus and influenza virus, and in this case, we have shown its relevance for generating a novel gene therapy adenovirus.”Bahou also sees a number of potential avenues for further developing the group’s line of research. “Certainly the possibility of using these reengineered viral systems for vaccine development is an obvious application. For applications directly related to this most recent manuscript,” he continues, “it provides a unique opportunity for safe methods for long-term gene delivery. On a broader application, one could envision entire cell systems with recoded genes as a means of altering cellular function in general. This is a forward-thinking statement, but certainly predicated on the status of genome synthesis coupled with progressive information on genetic pathways that regulate cell functions.”Those applications, he adds, will most likely feature the ability to rapidly characterize and synthesize attenuated genomes as new vaccines. In addition, “we are currently testing our ability to safely use this technology for gene replacement strategies which have been limited by concerns for insertional mutagenesis” – mutations caused by inserting genes into a genome. “Such an issue is especially relevant for benign diseases such as hemophilia where cellular transformation using integrating lentiviruses should be considered an unacceptable risk for long-term gene replacement strategies. Therefore,” Bahou points out, “our collective group is currently trying to apply this research as a safe means of long-term gene replacement. At the same time, Bahou stresses that “It’s important to note that genome reengineering using synonymous codon pair restructuring is a relatively new concept, and we fully anticipate that the applications will broaden over time. In theory,” he concludes, “we should be able to modify genomes for both attenuated expression and enhanced expression, depending on the situational requirements. In that respect, the technology is relevant for many other biological systems, especially when developing computational approaches to modifying cellular systems at many levels.” Viruses evolve to play by host rules Citation: Genes ex silico: Computer-designed virus yields phenotype expression benefits (2011, August 29) retrieved 18 August 2019 from https://phys.org/news/2011-08-genes-silico-computer-designed-virus-yields.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. More information: Computationally designed adeno-associated virus (AAV) Rep 78 is efficiently maintained within an adenovirus vector, Published online before print August 15, 2011, doi:10.1073/pnas.1102883108 , PNAS August 23, 2011 vol. 108 no. 34, 14294-14299 Functional characterization of single-backbone Ad/AAV/dRep hybrid virus. (A) Schema of Ad/AAV/dRep with predicted monomeric (M) and dimeric (D) forms resulting from productive Rep 78 cleavage of endogenous AAV integrating elements p5IEE and AAV TR (Fig. 2 shows excision model). Sizes are as follows: ψ, Ad packaging sequence (0.4 kb); pCMV/EGFP cassette (1.6 kb); pTK/NeoR, (1.1 kb); Ad, Ad base pairs 3,330 to 3,940 (0.6 kb); p5IEE, 135 bp; AAV TR (145 bp each plus G-C tail); and dRep, approximately 1.9 kb. (B) Ad/AAV/dRep was serially passaged in 293 cells, and at indicated passages (P), Hirt DNA was isolated for genomic analysis using the approximately 0.2-kb Ad left end inverted TR (ITR base pairs 1-194) as probe (horizontal bar). *Parent Ad/AAV/dRep virus. © PHAS, doi: 10.1073/pnas.1102883108 (PhysOrg.com) — Gene therapy is medicine’s rising star with adeno-associated virus (AAV) vectors – nonpathogenic parvoviruses – among the most promising supporting actors, due largely to their capability to integrate into transcriptionally silent genomic regions (areas that do not, via RNA polymerases, make a messenger RNA copy of DNA-stored genetic information). That being said, there’s been a downside in assembling AAV vectors into adenoviral (Ad) viral backbones, which are used extensively in genetic research and therapy: They rely on replication (Rep) proteins – in this study, the Rep 78/68 polypeptide – which limit viral amplification methodologies. Recently, however, researchers at Stony Brook University have used computational microbiology to reengineer the Rep gene through synonymous codon pair recoding, a technique in which the meaning of a codon (a set of three base nucleotides) is changed in a dynamic site- or mRNA-specific way – that is, it competes and shares expression with the standard. The experimental results demonstrate the value synonymous codon pair reengineering can have in modifying endpoint expression. Copyright 2011 PhysOrg.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed in whole or part without the express written permission of PhysOrg.com. Explore further